GrainGenes Sequence Report: BQ171601

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Sequence

BQ171601

Contig

NSFT03P2_Contig8293

External Databases

TIGR Gene Index

wEST map position

BQ171601

NCBI UniGene

Ta.48560

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Clone wle1n.pk0042.f7:fis, full insert mRNA sequence'

Keyword

EST

Species

Triticum aestivum

Cultivar

Chinese Spring

Chromosome

4AS

4BL

4DL

Clone Library

Wheat heat stressed flag leaf cDNA library

Tissue

Flag leaf

Developmental Stage

Full tillering stage

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1659-1662_I20_I20ZT Wheat heat stressed flag leaf cDNA library Triticum aestivum cDNA clone WHE1659-1662_I20_I20, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1659-1662_I20_I20

Probe

WHE1659-1662_I20_I20

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants at full-tillering stage were heat stressed under four conditions: (1) 38 C for 2 hours, (2) 38 C for 4 hours, (3) 2 days of cyclic treatment of 38 C for 2 hours, (4) 2 days of cyclic treatment of 38 C for 4 hours, at Texas Tech University (D Zhang in Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton, Malatrasi) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttggaggggaatctgatgctggctaagataaag
caaaagatgtgcaaccctgttacaatacagtgagaggaacatacttcacg
ttgcctgataacactctttgagctcaaaactgggcgctttgctgtgcact
atctgctttccagcaaccagcggtgagtttatcataaacagcagtgtgct
tcatcaagaatagttatagtacaggtgcccctgttggatccgcagaaaac
cgcaaatgatgaatattctagtagtgcatctgttctgcacaacctgtgaa
gcttcatgatgcaaaattacagcatctagccagactaacgaaaacaacgt
ggtacaaacacagctccagaagatcacaacaaaaatgcttggagcggcag
agattgtgcacgacatgactaactgga


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