GrainGenes Sequence Report: Ta.5161.1.S1

GrainGenes Sequence Report: Ta.5161.1.S1_at

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Sequence

BQ171551

Contig

Ta.5161.1.S1_at

Ta.5161.2.S1_a_at

NSFT03P2_Contig15562

External Databases

TIGR Gene Index

wEST map position

BQ171551

NCBI UniGene

Ta.5161

DB Remark

Locus Source: Triticum aestivum (bread wheat)

UniGene title 'Transcribed locus, moderately similar to NP_001067535.1 Os11g0225000 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Triticum aestivum

Cultivar

TAM W101

TAM W-101

Chromosome

4AL

4BS

4DS

Clone Library

Wheat drought stressed leaf cDNA library

Tissue

Leaf

Developmental Stage

Full tillering stage

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE1411_F03_L05ZT Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1411_F03_L05, mRNA sequence.

Strain

lab_host E. coli SOLR

Clone

WHE1411_F03_L05

Probe

WHE1411_F03_L05

Remark

DB_xref: taxon:4565

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttttttttttcgggagaataaaatatccattac
cacgaagagatatgtggagtacgtagcagttacaaggagaaatacaattt
aataggcttcacaatgacgtacatatcatagcgtactgttttataaataa
ttcattctagcaataatagggagtatccattccatcttgcacatgtctgc
tgaacaaaacccaaatttcatgctcgatggagattggagacctggcatga
cattttagtttcttatggatgcacatcgtccgtttagcttgtcgagaatt
gtttctatggatggtctcttcagccgatcaatctcaacacattccagtcc
tatttcaatgcatttttttacttgttggagggcatccacatctaacaatg
ggtactcagatgcgatgcgctgctctttccaattttcacgtacatttttt
atgaattgcactgc


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