Query (optional)   in Class  

GrainGenes Sequence Report: BQ171511

[Submit comment/correction]

Sequence
BQ171511
Contig
Ta.12065.1.S1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC294656
wEST map position
BQ171511
NCBI UniGene
Ta.17263
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001059669.1 Os07g0489800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
TAM W101
TAM W-101
Chromosome
7AS
7BS
Clone Library
Wheat drought stressed leaf cDNA library
Tissue
Leaf
Developmental Stage
Full tillering stage
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1401-1404_O09_O09ZT Wheat drought stressed leaf cDNA library Triticum aestivum cDNA clone WHE1401-1404_O09_O09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1401-1404_O09_O09
Probe
WHE1401-1404_O09_O09
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were given a gradual stress down to 65% and 78% RWC at Texas Tech University (D. Zhang in HT Nguyen lab). Total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close), at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttgaaccgattgttatatttgagcattggatcact
ttggattattttgtactatttgtttacgagcgcaaggaagtgcgcctttg
cttctagttctacgggacgccttaacatgtctctgtgccctttacatata
gtcctcgttgaggtttcaagcagacagcctctagttcttcttctcttgac
caagtgttgcccctgtctaattattccagggtcatgactcatgaggccta
ctgcataatcatcataatgttataatccagtcacttctcccacttgtgta
ccttccactctggtagctgctgtatgaccatctgtcctgttgaatctggg
ttgccaaccgtggcctggcacgccagcctccatgacttgggtttcctttt
gagcttttccttttctacttcgtttttcggagaaagcatttcgccgcctt
ccactacctcc

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.