GrainGenes Sequence Report: Ta.5063.1.A1_at
Sequence
BQ171429
Contig
Ta.5063.1.A1_at TaAffx.106383.1.S1_s_at NSFT03P2_Contig12096
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC287992
wEST map position
BQ171429
NCBI UniGene
Ta.5063
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001067149.1 Os12g0583900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
5AS 5BS 5DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1789_H02_P03ZY Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1789_H02_P03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1789_H02_P03
Probe
WHE1789_H02_P03
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gacaataaaactctacgtcaaccatgtaaccaactccttggcccaaacgg
ccaacaacagcaacgacggaagtcctagcatccttgtaccatatatccca
ctaagctgaaacctgtgaccgcaccaatcgctacgggccacagggggtaa
aaggctaacaagaacaaaggggatttacattgcacagcagcacaggataa
ttagcagcaccccttctagaatcagagaaaccggtgcggaaccaagtcat
ggacccgtatcgggtagctgtagaactcctcgttcctggggtccgagtac
cctttacggcggcgcagaaatggcgtgaaccatgagagccctcggtcgtt
ctcgttggcacggggctccagcgtgttgtccagaagcgacgcgacgatca
gcgccaccgttgggccggaggcaaaaactgtgttgatgatgtcattgaac
catcctgcatttgttcgggcaggtccgcggccagcagaagcggtgtactc
tgcgaaatattgagggatcgagatgccaaggaacaaggatagtccaatga
tgtagatgttcctcatggagtttttgtttgcaaactggctgaacgagatc
ccacagcagctacgataccaaac
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: Ta.5063.1.A1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||