Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Sequence Report: BQ170444

[Submit comment/correction]

Sequence	BQ170444
Contig	Ta.11606.1.A1_at Ta.913.2.S1_s_at NSFT03P2_Contig6265
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC361476
wEST map position	BQ170444
NCBI UniGene	Ta.54056
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	4AL 7AS
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1772_F04_K08ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE1772_F04_K08, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE1772_F04_K08
Probe	WHE1772_F04_K08
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttaactgattatagtgatagtgttgtctggtgc agataatattatgcggtacctgatacagttctcatcgagctaagctgcta gctagcttattacgaaattgtaaatttttttgggggataattaacagtag tggaggccggtcgatctcgcgctgcacctttctccgtccgtctgtccgtc catggtgaccatggctggtttagttgcagcagcagcagaggcaatcgagg cagcactcacaggtctcgtagcagcagaagcagcacagcgccgtgaacac gctgcaaatgaaacacccaattatatcagtcaaacaataatagcagcatc atagaagggaagaatgaacagaacacaaatgaattaacaggaaccggtac tagcctactaggggaatcagtcccaagatattttcctgccctgtctctat gatttctccgaggatttttcagatggcagaaaagtttatctct

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.