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GrainGenes Sequence Report: BQ170045

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Sequence
BQ170045
Contig
Ta.30645.1.S1_at
Ta.30645.1.S1_s_at
NSFT03P2_Contig9265
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC305183
wEST map position
BQ170045
NCBI UniGene
Ta.21040
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Homologous to ALY of mouse'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7AS
7BS
7DS
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0987-0990_L21_L21ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0987-0990_L21_L21, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0987-0990_L21_L21
Probe
WHE0987-0990_L21_L21
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttgttttttttttttttaaatcatcagaagaaaa
atactaaacggcaacagccacccgaaaacaaatggttgctcaaagctgtt
aaccaccaattacaatgttactgctggactagaactaggaactgcagttc
aaatctacggttttcaagcttgatgtaaaagacatgtaacatatgtgcac
acaactgggagatgaccaacggcaactcggcagcaaaccacacataaaac
aagctcaaattcaaagcctcagccaagttctaggttcaatgtgataagat
gcagcatcttagtcaacattcattgctccagaatgataggtgtccagctc
cttgtccaattggtcagccgatttcaccaaccccttccgtacat

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