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GrainGenes Sequence Report: NSFT03P2_Contig12164

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Sequence
BQ169881
Contig
Ta.20248.1.S1_at
NSFT03P2_Contig12164
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC294863
wEST map position
BQ169881
NCBI UniGene
Ta.20248
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Holocarboxylase synthetase'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AL
3BL
3DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0976_A02_A04ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0976_A02_A04, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0976_A02_A04
Probe
WHE0976_A02_A04
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttaatttaaccctctcattgtattaagcacataaactctttgctat
atcatagcaattttcatttacattcagaatcttcatggcaaagccccaaa
aagattgttgggatataagacatgaaccaggcatacatatagatttctct
ccaaacaggcttgaacaaaagaaaacaaatccaataactttaagtgaatc
gccaggtgtgcaactataaagccacacagtgcatgagctttttaggaaaa
atacataacatacaaaaaccagagaaaatactggcactcaatgcttcctg
gttgtgcatgcaacatgtttccagaaactgacttagtagtacccatttcc
cgcgctgtctgaaaccatgtccatggaggtatcattggagcttgcagagt
tggcctctcggttttgcccggctgagtttgcttc

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