GrainGenes Sequence Report: NSFT03P2_Contig11402
Sequence
BQ169778
Contig
Ta.11286.1.A1_at NSFT03P2_Contig11402
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ169778
NCBI UniGene
Ta.48608
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Hypothetical protein LOC778393'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS 4BL 4DL
Clone Library
Wheat pre-anthesis spike cDNA library
Tissue
Spike before anthesis
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0969_A05_B09ZT Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE0969_A05_B09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0969_A05_B09
Probe
WHE0969_A05_B09
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttttttgaagcccaatgtaatttttattatgc
cttgtacttctcgtgagcttttataaagatccgagttcttgtcacaaaag
aaaaagaatccatcgtcaaattcacaacatgatcatcacacgaacccaat
aaacaagcaaaccataaactgatacacctccttgctaatataaaaccagc
aaatcaaatgagacactacacactctagtccaacggataagggatcgtgg
cgatgtggttgtgcgcaacggtcccgatccacagcctccggtccacctcc
ctcacctcgctcaccagcttcatcacctcgccgccccggtcctcgagtac
ctcgaccacgttcccctcgccgtcgaggagcgcgagaagcgtgtacatct
tcatgctcaccatct
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: NSFT03P2_Contig11402
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||