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GrainGenes Sequence Report: NSFT03P2_Contig8447

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Sequence
BQ169074
Contig
NSFT03P2_Contig8447
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BQ169074
DB Remark
Locus Source: Triticum turgidum
Keyword
EST
Species
Triticum turgidum
Cultivar
Langdon-16
Chromosome
6AL
6BL
6DL
Clone Library
Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library
Tissue
All tissues
Developmental Stage
Different growth stages
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2155_B01_C01ZT Triticum turgidum L. var. durum (durum wheat) whole plant cDNA library Triticum turgidum cDNA clone WHE2155_B01_C01, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2155_B01_C01
Probe
WHE2155_B01_C01
Remark
DB_xref: taxon:4571
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in a growth chamber at North Dakota State University (Kianian, Otto, Simons). Tissues collected from seven-day etiolated seedling leaf, stem, root and seed; leaf from plant at fourth leaf stage; spike from pre-anthesis through 20 days after anthesis; flag leaf; leaf and stem tissue from tillers. and root. Total RNA and poly(A) RNA were prepared from each tissue and then pooled, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttgtcccaaaaaataagataaaaaatatgcgatca
tccgatccaaatcaccacaagtccacaacatataagcagaaaacgacgga
gaatctgaaatttttgctcctatatcgcagaaaagaataagtaaacgact
cttccccccagagaacaaaaccgtttcgtccaagtgttgcaactaattat
aggttacatatatataacagatttgacaaaaataaccagacagcaaaatg
tacaaatttttcttaggatgagagtggggtgcaggaagatccgagtgttg
cgcttgtccgct

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