GrainGenes Sequence Report: TaAffx.98058.1.S1_at
Sequence
BQ168892
Contig
TaAffx.98058.1.S1_at NSFT03P2_Contig10768
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ168892
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Species
Triticum monococcum
Cultivar
DV92
Chromosome
1AS 1BS 1DS
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2473_E02_J03ZT Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2473_E02_J03, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
WHE2473_E02_J03
Probe
WHE2473_E02_J03
Remark
DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttaacagctccactggactatatgcatttccga
gattggccggcgactccaaaaatacagggcagacaaacaaaaggatggtg
gaaatggaggacgcttatttggttcctacctaaaagcttccatggcattt
ttcctcttttcttctcatcatctggggaggctgtggaggtccaaaatgtg
agctcactggcaatgtccgtcttgtgtgtgtgtgtgttctgcctcggtca
cagatcgtgctcgggcttgtccatcgcagcgctgtcgccgctctcggcca
tccggctcgagccgcgggcgatcttggagatggccgaggacagccccttg
aagctgctgctgccctcactgaggttgtgagagcgccgggaccgccggtg
cgcgagctgcggggaggctggcctgtcggggtcgccgccgtctgctgtcc
gggtctgcgccaccaccagccgcgagatgctgccgcggcagaacgggcaa
gccggcgagggcagggtgagcgtcgccgggttgggcttgttgtggc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: TaAffx.98058.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||