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GrainGenes Sequence Report: Ta.25896.1.S1_a_at

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Sequence
BQ168851
Contig
Ta.25896.1.S1_a_at
NSFT03P2_Contig11922
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
wEST map position
BQ168851
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Species
Triticum monococcum
Cultivar
DV92
Chromosome
3DL
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2471_B11_C21ZT Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2471_B11_C21, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
WHE2471_B11_C21
Probe
WHE2471_B11_C21
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttgaagagtagtttggtaataaaaagacactata
tggcttaaaagtagatccaatttgttgaaggcatcatcaagcgatggcga
catgtgaaagtacaattgcacttggcacctgtggcgcggcagaagatgat
aacattgtaaaggggcatcatcttaatggcttcagtcccttggaggctcg
cagttcatttgcagccgctcggagagaatcatctgatagaattaaccttg
acagggcaccagtgcttaggcctagaattttcgctgcctctgatacagaa
ccttcaacagcaaatagaagatctaataaagcttgcattcctggagaaaa
ttttgggttatttggcccaatttggctgccaacatcctttgatcgaa

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