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GrainGenes Sequence Report: TaAffx.12928.1.S1_at

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Sequence	BQ168846
Contig	TaAffx.12928.1.S1_at NSFT03P2_Contig11914
External Databases	Data at GenBank Data at EMBL Data at DDBJ
wEST map position	BQ168846
DB Remark	Locus Source: Triticum monococcum
Keyword	EST
Species	Triticum monococcum
Cultivar	DV92
Chromosome	4AS 4BL 4DL
Clone Library	Triticum monococcum early reproductive apex cDNA library
Tissue	Early reproductive apex
Developmental Stage	Seven week-old plants
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2467_G11_M21ZT Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2467_G11_M21, mRNA sequence.
Strain	lab_host E. coli XLOLR
Clone	WHE2467_G11_M21
Probe	WHE2467_G11_M21
Remark	DB_xref: taxon:4568 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	gcgacttcatcctcagaagccagccgagccgcagcagaggcacagagcaa atcaaagttgttacatttgcaaaggacaaattctggcatgaataccactc agaaagttgatccaggggaaccagctgctaagattgctcaacaagcctct cagttcaaacgatggggacgcagacatccatttgttcgatatggtcttcc actcatttcgttgacagtgtttggtgcggttggtctagctcatcttatac agggaagcaaagaagtaacaaaggaaaaggaggatatggaatgggaggtc gtagagaaaacaaaagctctgagccgaacaggaccggttgaaggggccta taagcctaagaagctctcactagaggatgaactcaaggctttgcagcaaa aggtggacataaacagctacgactacaagagaattcccaaacaaaatgaa aacaagtgaagtaaagcaccggttatcatgctaaggagtgggggattttc ttaaaagctctgaagtagtttcttcatattgttacttcataagttccttg ggatgctgtcgaag

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