GrainGenes Sequence Report: BQ168658

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Sequence

BQ168658

Contig

Ta.4599.1.S1_at

NSFT03P2_Contig8103

External Databases

Data at GenBank

Data at EMBL

Data at DDBJ

DB Remark

Locus Source: Triticum monococcum

Keyword

EST

Species

Triticum monococcum

Cultivar

DV92

Clone Library

Triticum monococcum early reproductive apex cDNA library

Tissue

Early reproductive apex

Developmental Stage

Seven week-old plants

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

WHE2453_E04_J07ZT Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2453_E04_J07, mRNA sequence.

Strain

lab_host E. coli XLOLR

Clone

WHE2453_E04_J07

Remark

DB_xref: taxon:4568

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).

DNA

tttttttttttttttttttaatacttcaacgttctaatcttaaaacaagc
ctgatactttttttaagaatcacaacaagccaagacacgagacatatcaa
atgatatcagcatgcgtatatatatcaatctaaccggacggccaacactt
cttcaaaatcgctaacaaagtgtagaattgacgttcttatatctcaaggc
agacatctgcagcccttcaaagatcggccctcccagggtatcttgggaat
gggatgacatctctgatgttctccaggccggtggcgaaaaggatcatcct
ctcgaaccctaggccgaacccgctgtgcttcactgagccaaagcgtcgga
ggtccaggtaccactcatacggctccaaaggaagatctgcgtccagtatc
ctttgctta


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