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GrainGenes Sequence Report: NSFT03P2_Contig11972

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Sequence
BQ168448
Contig
Ta.6366.1.S1_at
NSFT03P2_Contig11972
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC319958
wEST map position
BQ168448
NCBI UniGene
Ta.50530
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001055459.1 Os05g0394200 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL
1DL
Clone Library
Wheat salt-stressed crown cDNA library
Tissue
Crown
Developmental Stage
Adult plant
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2108_C07_F14ZT Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2108_C07_F14, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2108_C07_F14
Probe
WHE2108_C07_F14
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aagacatactactacacataccgcaaaaaaggctaggcagacaatacaag
cattacaacattctggcgggggttttagctagcaccctaacaactcctgt
acatgttcttcatctagttgttaccactaccttgatcctgcatgagtgca
ttcagtctcagcttcgagtgcatttcggcagtagcatgggagaagtcggt
cagggcacggaacagctccggcaggtgcgccctcaggatcgaaagggatt
tctcccgaacttgcttcgacagcttgttatgggcctcgacctcgttatcc
agcttgcttttcaagcttt

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