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GrainGenes Sequence Report: BQ168391

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Sequence	BQ168391
Contig	NSFT03P2_Contig18091
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC284448
wEST map position	BQ168391
NCBI UniGene	Ta.28340
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'USP family protein'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	1AS 1BS 1DS
Clone Library	Wheat salt-stressed crown cDNA library
Tissue	Crown
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2102_E11_I22ZT Wheat salt-stressed crown cDNA library Triticum aestivum cDNA clone WHE2102_E11_I22, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2102_E11_I22
Probe	WHE2102_E11_I22
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions at UC Davis, salt stressed for 12 hours, and for 7 days, then dissected and frozen (Akhunov in J Dvorak Lab). Total RNA and poly(A) RNA were prepared from crown tissue, equal portions of RNA were pooled from the two treatments, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Akhunov, Chin, Choi, Close, Fenton, Kianian, Otto, Simons, Zhang). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttacacacacctggggaatcatccacaggctaattgtcaattattatta caaataacactggatggttcaataagttgcggccaactggatatatagga ctctggcaaactaaaacacatgtggacatttgcgatgatgagatcaggca tggttactcgctggcttgacaacggtgacagggcaggcggcattgttcac gacgtaatcgctgacgctgcccaagagcaccctcttgagcttgccaaggc ccctgctcccaatgaccagggagctgatgggcatgtcatggatggcttgg cacagcttctcgcggggatctccaaacaggacttttgaaaccacggagac ctccttctgcttagctatagtgttgagcatgtccagcgtttcagcatcag gcttcaccccatatttctttgcagttgaagggtgagagaactccgagagg gggatcaaaggcgagccggaggcctcccagaggagggtctcgccctgctc gtagtcggggtccttgaggacgtgcaggaggagcaggttgtcgccggagc ggagcaggttgtccgccgcccact

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