GrainGenes Sequence Report: BQ166599
Sequence
BQ166599
Contig
Ta.10804.1.A1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.10804
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Clone Library
Wheat 5-15 DAP spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0904_B05_D10ZT Wheat 5-15 DAP spike cDNA library Triticum aestivum cDNA clone WHE0904_B05_D10, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0904_B05_D10
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes at 5, 10 and 15 DAP were harvested, total RNA and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttttaaaaaaaaacaacttcctgtt
ggttgcctttttcttttcttgattgatcgaaccaactagagcaccgacaa
tgatctaaagtacagcgtgtttgttcaacacccaccatccatctagtaca
caaacccaaaaaaagccgccccaccctcaataacacagcgaaaaattaat
tcccgttgagtacagctcatacctctcaaaccgccatcggtgtgggggta
cataaagcgacgggcaccagc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ166599
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||