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GrainGenes Sequence Report: NSFT03P2_Contig12502

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Sequence	BQ166313
Contig	Ta.10695.1.S1_at NSFT03P2_Contig12502
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC308578
wEST map position	BQ166313
NCBI UniGene	Ta.58044
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001051247.1 Os03g0745700 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5BL 5DL
Clone Library	Wheat vernalized crown cDNA library
Tissue	Crown tissue of seedling
Developmental Stage	Five-week old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0842_E01_I02ZT Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0842_E01_I02, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0842_E01_I02
Probe	WHE0842_E01_I02
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttgacaagacgaccagtgtttatgcaaagcaaa ataaaccatcatctcacccacaaccacaagcgagtagcggcagtaggcaa cctacacatttcttcttccacgagagacgccgcgacgaccaaccaaagcg ttcacccatccgcacaaccgcaccaccgaaagggaaacaaaaaacagcag caggccgacccgcatcacaccacaacgagtactacttggagaagccgaag cagctgcagccctgcgaggtgtggtcggggacggcgggtgtcatgctctt gcccttgntggtggtcgatcctctccagcatcgccaccacctcggccatc tccggccgggtggtccgggttgccgtcccagcaccgcgtcatgatctccg acagcggcttggggcagcaccgcggtatgtcgggccggatgcccagcttc accacgtggtacgagatgtccgcgatgctgtagttcgggtacgccaaggc gcagcagtacgtctcccacagcaggacgccgaagctgtacacgtcgcact tgtgggtcgtacgggcgcccctccagcactcggggggcatgtaccccagc gtgcccgtctgccccgtcat

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