Query (optional)   in Class  

GrainGenes Sequence Report: BQ166053

[Submit comment/correction]

Sequence
BQ166053
Contig
Ta.10589.1.S1_at
NSFT03P2_Contig5656
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC329724
wEST map position
BQ166053
NCBI UniGene
Ta.10589
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
7DS
Clone Library
Wheat vernalized crown cDNA library
Tissue
Crown tissue of seedling
Developmental Stage
Five-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0807_A04_B07ZT Wheat vernalized crown cDNA library Triticum aestivum cDNA clone WHE0807_A04_B07, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0807_A04_B07
Probe
WHE0807_A04_B07
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were germinated and grown at 4 C for 5 weeks. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttttgaagataacatttccatatatttatttttg
tctcatggatcgatccgttgacattttattagcacatcacagcacagaca
cgcacaatacatgcagacataaagggcttacaagttactccctccgttcg
gaattacttgtcacaaaaatgcacgtgtctacaactaaaaaccatctaga
tacattcattttttcgacaagtaacttcgaatgaaagtagtacaatatag
tgttggctctgacagacgcagcggcggcgggcgcatgtggcgtcgccagt
tcgcagccgggcctagcagcccacgccggagtaggtgcactcgcatgtgg
ccttgcaggccggcggggtcgtgcacacggcggggcagaacttcccttcc
gggcagggcacacacccgcagcggtcgatgcacgtggctagcccgtcatc
agtgggctgccccgatgtggccg

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.