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GrainGenes Sequence Report: BQ162775

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Sequence	BQ162775
Contig	Ta.9017.1.A1_at NSFT03P2_Contig8725
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC294268
wEST map position	BQ162775
NCBI UniGene	Ta.45191
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001055174.1 Os05g0316200 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	2AS 2DS
Clone Library	Wheat etiolated seedling root cDNA library
Tissue	Root
Developmental Stage	Five day old etiolated seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE1213_E08_I15ZT Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE1213_E08_I15, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE1213_E08_I15
Probe	WHE1213_E08_I15
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttttttttttttttttttttttttttttgatccactgttttctcattat tatttttataaccaaatatgaattattttctacacaccaaacaatcggca cagccgcgctgtacatgatccacaagcccaaaatgtacatacagaagctt ttgtgctgtaacactacagacaacggagcacatctttactgttacacacc agaaatcacactgcctctctacagttacacacaagttttgatagtatgaa cattggacaccacaaaagaagatggcacacacatgcactagttccccaac cattcgaagcaacggcaaaggccccagcaaaacttcgaaggatagtcccg tatgaaaacatccctgccgatagccgacaacgtagaagctaaaagaccgc ttacggtgagacattgttacacgggttacgggcacaactgagatgatgtg cctctccgaaatcactcttgcagatggtggaactggcagccggct

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