GrainGenes Sequence Report: BQ162756
Sequence
BQ162756
Contig
Ta.3552.1.S1_at NSFT03P2_Contig11516
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC294191
wEST map position
BQ162756
NCBI UniGene
Ta.48695
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wre1n.pk157.g3:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
3AS 3BS 3DS
Clone Library
Wheat etiolated seedling root cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE1212_B08_D16ZT Wheat etiolated seedling root cDNA library Triticum aestivum cDNA clone WHE1212_B08_D16, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1212_B08_D16
Probe
WHE1212_B08_D16
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttttttttttaccacagtcaacaggaaattccc
cgtgattcaaacaggccctaaatgaagagggcaaaggtatcatctgcatt
acagggaacatttattacaggggaaactgctgtatcatctgcattcttgt
agcgctgattccttgggctaaaaattaccctaaaacatcacaaaatcaat
taggggaatgagcataataagatggccggaactcataccggttgcaacgg
ccctgtttgatttctggaggtgataacatgcagtaaggaagctattgata
gcgggttcttgatatttacacctgtagttagttagattgcaacgttcttt
gtccctgctccacttgcagagacttctcaaagttcaccagctgggatata
aagcccgcattgggtgctatttgaggtcgtttgcttctaactagtgacat
ggcactttgcaggctcatctgatgcttcttca
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ162756
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||