GrainGenes Sequence Report: BQ162350
Sequence
BQ162350
External Databases
Data at GenBank Data at EMBL Data at DDBJ
wEST map position
BQ162350
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AL 3BL 5AS
Clone Library
Wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0752_B11_C22ZY Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0752_B11_C22, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0752_B11_C22
Probe
MAG1294 WHE0752_B11_C22
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A, (T; )27G and (T)27C). Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: Oligo dT wobble primer (an equal mixture of (T)27A,; (T)27G and (T)27C). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cactttataaaaataataataatgatatggcagcgaccaaacatcagtcg
ttttgacgcgtgtttgcaccggctgaatattacatgcaccatattgtgct
ccccactttgtcccctcctccgactcttgtgaccccctccaacgccaacc
tatcctccaccccgcgcaccgccacacgtgttgccccaccccacccacaa
atcgcaggtcgaatacgtggcgccacctgaacatctgcaggaaccataca
ttcaacattccttgtaaacgatcatccaccacacgcagatccacccgatt
cttgttaactggccattcagcctgtactccttcacaaagtcttctccctc
aggattataagatttggataagtatgcttgaaccacttctttgcaacatc
cgttgatgtgaatgatagaattttgtggtagaaactaagcacgtcccttg
ggttcggctcatcaagcctcaatccagcttgcctatggaacttatctccc
atatcacctttaactatgttcttgaccgtatccacttgcaacttc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BQ162350
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||