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GrainGenes Sequence Report: NSFT03P2_Contig10161

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Sequence
BQ162332
Contig
Ta.9746.1.S1_at
NSFT03P2_Contig10161
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC361995
wEST map position
BQ162332
NCBI UniGene
Ta.9746
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001049713.1 Os03g0276600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
4AS
4BL
4DL
Clone Library
Wheat heat-stressed seedling cDNA library
Tissue
Leaf
Developmental Stage
Two-week old seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE0765_G02_N03ZT Wheat heat-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0765_G02_N03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0765_G02_N03
Probe
WHE0765_G02_N03
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Two-week old seedling were heat treated at 37 C for 2 hr and cyclic heat treated at 37 C for 2 hr for two days at Texas Tech University (D. Zhang, H. Nguyen lab). The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ttttttttttttttttttatggtgacatacaaacctaactgttgattctc
aatcgaatgatgtcagtttacaatgcaaaccatctacaaaaatggattca
agttatattcttcagatgatttatattatcataactgaagatctgaatct
tgtgccctttggtttgagaaagagctactatccccttcaacctaatccgc
tatagctctttacgctgtgcagagcttcttcttctagctcaaccaacatg
cggcagaagtcttcaacatccttgactgcttccttgaatgtgaacctgaa
ctctttccaatggtactgctgagggacatcaggtgcggtagctctttcag
gaaccctggcgagcacctcaaatccatggtgatcaacggagagcaagagt
gcatctctggcagtcacatcaaaatactcgaaacagaaaagtataagtgc
tctccgccttgggtcactcctgttgataccatcaataagcctcgaagaga
atggcaatagcaggtcggcttttggtggttcaaaagtatccaattcgata
ttatggctgctccccaatgaatctatgaagacacacgagaagtagcactt
gcggaagacaataccgttgaaaccccataagtagcttcgctagcagcttt
atgt

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