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GrainGenes Sequence Report: BQ161309

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Sequence
BQ161309
Contig
NSFT03P2_Contig15595
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC303888
wEST map position
BQ161309
NCBI UniGene
Ta.40147
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001054517.1 Os05g0125000 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Chinese Spring
Chromosome
1AS
Clone Library
Wheat etiolated seedling root normalized cDNA library
Tissue
Root
Developmental Stage
Five day old etiolated seedling
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE1101_A05_A09ZT Wheat etiolated seedling root normalized cDNA library Triticum aestivum cDNA clone WHE1101_A05_A09, mRNA sequence.
Strain
lab_host E. coli DH10B
Clone
WHE1101_A05_A09
Probe
WHE1101_A05_A09
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Roots were harvested. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. The cDNA clones were in vivo excised to give pBluescript phagemids before normalization was carried out. The mass excision of phagemid library and normalization were done in HT Nguyen lab by D. Zhang at Texas Tech Univeristy. Normalization protocol used was that of Soares. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gggccccccctccgagcggatagggaaacagttcagagatcagaatatga
tctccaggtttggtgcattcttatgaatgataaagttcagttcaggatgc
agtggccccaatatgcagaattggaagtgaatggttttgctgtacgagta
gtgaccagacctggttctcagttgctaggcataaatggacgggatgatgg
gccattgataaccacatgcagtagagaaggaactaacaagatctgcttac
gaagggttgataatcgaacattttgctttggagtccgagttgctaagagg
aggagtgttcctcaggttttaaatttagttccaaaggaagctgaaggtga
gtcttttgaggatgctcttactcgtgttcggcgctgtctcggaggtggag
atactgcggagaatgctgatagtgacagtgatttggaagtggttactgaa
tctgttacagtcaatcttcgttgccctaatagtggatccagaatgaagac
tgctggaaggttcaagccttgcattcacatgggttgttttgatctcgaca
ccttcgtggaactgaatcagcggtcacgcaagtggcagtgtccaatatgt
ttaaagaactactctcttgagaatttgatgattgatccttacttcaatcg
gattacttctctgttgcgtgattgtagtga

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