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GrainGenes Sequence Report: BQ161238

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Sequence	BQ161238
Contig	Ta.633.1.S1_at Ta.633.3.S1_at Ta.633.3.S1_s_at NSFT03P2_Contig2667
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC293156
wEST map position	BQ161238
NCBI UniGene	Ta.48294
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001066971.1 Os12g0548300 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Chromosome	5AS 5BS 5DS
Clone Library	Wheat cold-stressed seedling cDNA library
Tissue	Seedling
Developmental Stage	Five-day old seedling
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE0368_E06_I12ZT Wheat cold-stressed seedling cDNA library Triticum aestivum cDNA clone WHE0368_E06_I12, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE0368_E06_I12
Probe	WHE0368_E06_I12
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized , germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Seeds were surface-sterilized, germinated and grown aseptically in the dark at room temperature on filter paper with water, nystatin and cefotaxime in covered crystallization dishes. Five-day old seedlings were transferred to 5 C cold room and kept for 48 hr. The tissue, total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	ttttttttttttttttttcgtccagttctcaacaggaagctttatactga ccaaaggccagagttctgaacccgctcaacattacaaagaaagaaagaac atacaggtgagcaatggtgaaaattttggagccctgactggctactttac ttggtagaagtaaactcatacatggccccctcagaatcaagttgaccaaa taagtattggatcatggttcttgcagtgatatgcaaagatcactctataa gccagggtgtttgaacct

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