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GrainGenes Sequence Report: BQ160146

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Sequence
BQ160146
Contig
NSFT03P2_Contig7284
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC5119
wEST map position
BQ160146
DB Remark
Locus Source: Secale cereale (rye)
Keyword
EST
Species
Secale cereale
Cultivar
Blanco
Chromosome
4BL
4DL
5AL
Clone Library
Secale cereale anther cDNA library
Tissue
Anther
Developmental Stage
Adult plant before anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankDownloaded 2008-2009
Title
WHE1272_H09_O18ZT Secale cereale anther cDNA library Secale cereale cDNA clone WHE1272_H09_O18, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE1272_H09_O18
Probe
WHE1272_H09_O18
Remark
DB_xref: taxon:4550
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; This EST was generated by sequencing from the 3' end of the clone.; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20.; Seq primer: T7 primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Anthers were harvested and pooled from early meiosis to late meiosis. The tissue, total RNA, and poly(A) RNA were prepared (Butler, Ross and Gustafson) at University of Missouri, Columbia. A cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
tttttttttttttttttttacggcggcacaggcgtggttcctttcccaga
ggttgtaaaaaagtcatacacattgtagtacatggttctggaaagaaata
tgttgtgatgcatacctgcactgcacgtatgtaagttgggataacgtttt
cttgacagtaccactagctaacaaagacaaggagaaagtgtgggagatga
atttacttcttctaagcaaccacacagaaacaaacccaaaattaaagtaa
cactctcgacaccggacacgagctaattagaaactgaggccgagctgcag
cccaaacgcggcatggaaaaggaagagcgtcatgatgctgctgccgagca
ggccgtgcgcgcctctcagcccagggtttccctcaaacaatgttggcagg
agagactgcacggtgaggagcgcaaggccgatgacaccggtgaccgcgtg
agggctttcgaagatgggtttaccggaggtga

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