Query (optional)   in Class  

GrainGenes Sequence Report: BM442869

[Submit comment/correction]

Sequence
BM442869
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC157217
NCBI UniGene
Hv.3855
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf28l24, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
root, 3 week, hydroponic grown, low nitrogen, cv Optic, EBro02
Tissue
root
Developmental Stage
3 week
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBro02_SQ001_K05_R root, 3 week, hydroponic grown, low nitrogen, cv Optic, EBro02 Hordeum vulgare subsp. vulgare cDNA clone EBro02_SQ001_K05 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBro02_SQ001_K05
Probe
kr32C1105
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: On Feb 1, 2002 this sequence version replaced gi:18473644.; Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from roots of 3 week old Nitrogen stressed barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
gcgcagccgtcttcctcctctcgctccctctcctcttcttctcctgttgt
ggttgtggttgtaggtaggtcgggaacgggaatcgatggcgctgctcaag
gtttacgcggaccgtctttcgcagccgtcgcgcgcggtgatcatcttgtg
caaggccaaccggatcgatttcgaggaggtcaaagtggatctggcaaagg
gacaacaccgacgccaagaattcaacaagatcaacccgatgggacaggtc
ccagcaatcatcgatggaagattcaaactattcgaaagccatgccatcct
gaggtatctcgccaccgtcttccccggagtcccggaccactggtatccag
ccgacttatttaccagagctaagctcgagtccgtcttggattggcatcac
tctaatctgcgccgtggtgcagcatcctatgtactgcacaccgcgttggg
tcctgctctcggtctcacacccaatcctgagaccgcaaaagagggcgaga
agttgctgtcaaaatcactgggaacgatcgaaa

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.