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GrainGenes Sequence Report: BM375750

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Sequence
BM375750
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC168292
NCBI UniGene
Hv.22884
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf103p23, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
embryo, 21 DPA, no treatment, cv Optic, EBem06
Tissue
embryo
Developmental Stage
21 DPA
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBem06_SQ004_E23_R embryo, 21 DPA, no treatment, cv Optic, EBem06 Hordeum vulgare subsp. vulgare cDNA clone EBem06_SQ004_E23 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBem06_SQ004_E23
Probe
BaGS28O21
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: On Jan 10, 2002 this sequence version replaced gi:18119140.; Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (21 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
caccgctcctccaacttccacaatcccctacagctcgaagaggatcgtca
gccatgtccgggcgcggcaagggaggcaagggtctcggcaagggcggcgc
caagcgccaccgggaagtcctgcgcgacaacatccagggcatcaccaagc
cagcaccacgtcgcctggcgaggaggggcggcgtgaagcgcatctcgggg
ctcatctacgaggagacccgcggcgtcctcaagatcttcctcgagaacgt
catccgcgacgccgtcacctacaccgagcacgcccgccgcaagaccgtca
ccgccatggacgtcgtctacgcgctcaagcgccagggacgcaccctctac
ggattcggcggctaggcccctcgtcgtcgccagtcttcgtcgccgcctgc
agattccgaatcttagttgaatctgccttgtttagttcgctccccctctc
tgtatgttgaaatgtatcgtggagcaaagtcattacaatgtggagttggg
aatcacacttgtctctccaaaaaaaaaaaaaa

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