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GrainGenes Sequence Report: BM372927

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Sequence
BM372927
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC187412
NCBI UniGene
Hv.3946
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transmembrane protein mRNA fragment'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
maternal, 10 DPA, no treatment, cv Optic, EBma04
Tissue
maternal
Developmental Stage
10 DPA
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBma04_SQ002_G19_R maternal, 10 DPA, no treatment, cv Optic, EBma04 Hordeum vulgare subsp. vulgare cDNA clone EBma04_SQ002_G19 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBma04_SQ002_G19
Probe
BaSD15M02
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: On Jan 10, 2002 this sequence version replaced gi:18116317.; Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from maternal tissue dissected from developing grains (10 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
ctgtctcgtcagtagtaatatcaaaacttctcagtgagcaatggcaaaga
gtctcgccgtcgcgctcctgctactggtggtgctcgcgtcatgcgacggg
cgggagctgaaagagaaggttgcagcgacacacggtgccggtgccagcgg
tggcgtcggcgactccaaggctttggacttgccgctgctggggggaaccg
gtaccggaacaagcaccattaccggcccgctggtggtgatccctgggatc
cctgctatcggcccatgatcatctctacctcatgtgctggcgctacaaga
agctaaagctcgccttgtcgctttagattctataggtagcaccgccagga
agaaaaaggccgacgtttcgcgaatgtccatgttcggtactgagcgtctg
tgtcaactgtcatttgtatttatttccagttcccgtctcgtatttttcgt
tccttgtgcgctacatgtatcaacaatgacataataaatacgtactatgt
tagtgtgaaaaaaaaaaaaaa

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