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GrainGenes Sequence Report: BM372629

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Sequence
BM372629
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC185784
NCBI UniGene
Hv.15180
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Methionine synthase 2 enzyme (ms2 gene)'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
pistil, 8 DPA, no treatment, cv Optic, EBpi05
Tissue
pistil
Developmental Stage
8 DPA
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBpi05_SQ003_H23_R pistil, 8 DPA, no treatment, cv Optic, EBpi05 Hordeum vulgare subsp. vulgare cDNA clone EBpi05_SQ003_H23 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBpi05_SQ003_H23
Probe
bags1h11
BaH47G19
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: On Jan 10, 2002 this sequence version replaced gi:18116019.; Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from pistils dissected from developing grains (8 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
cgccgacgtcatcaccattgagaactcacgctccgacgagaagctcctct
cggtcttccgcgagggcgtgacctacggggccggcattggccccggcgtc
tacgacatccactcccccaggatcccctccacggaggagattgccgaccg
cgtcaacaagatgctcgcggtgctcgacaccaacatcctctgggtgaacc
ctgactgcggcctcaagacccgcaagtacaccgaggtgaagcccgccctg
actaacatggtcgctgcggccaagctcatccgcacccagctcgccagcac
gaagtgaggagccatgaaatcgaacagcctgatgatactactacctattg
ttttgtggggacagagcgccaaacgagccgtttgtttcgaataatccgat
tctttatcgtaccgcccgagtctggctagggttagtcctgctgttatggt
aaaactgttacatgttgatggcatacgttgtattctatgtgtttgggtgg
gtggtctcattgtttacaggttgtgtggtnctgtcgcggttataaacaga
gttgaatgaaaattgttctgcanaaaaaaaaaa

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