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GrainGenes Sequence Report: BM369630

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Sequence
BM369630
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC157913
NCBI UniGene
Hv.6657
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001056190.1 Os05g0541900 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
embryo, 28 DPA, no treatment, cv Optic, EBem07
Tissue
embryo
Developmental Stage
28 DPA
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBem07_SQ004_C20_R embryo, 28 DPA, no treatment, cv Optic, EBem07 Hordeum vulgare subsp. vulgare cDNA clone EBem07_SQ004_C20 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBem07_SQ004_C20
Probe
baak21e08
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from embryos dissected from developing grains (28 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
ccggcccttgcaaggctgagcgcagtgtaccgcagcctccaggtttccaa
gtctggggtcaagaagaagaacaggcagccaactaagctgtaaactcatt
aaggttgccttttggcactcatgttatcatgtctgttccaacagttttga
gtcataagtgagacccttggaagaaccatgttacaaatccgccctagttt
gtaccctgacagtgttagaagaatggtttgtgttgacctataatcgctgc
ttgttgtta

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