GrainGenes Sequence Report: BM137381
Sequence
BM137381
Contig
Ta.27350.1.S1_at NSFT03P2_Contig9885
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC330205
NCBI UniGene
Ta.48670
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Clone wlm96.pk035.d7:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
Wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE0463-0466_C23_C23ZS Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE0463-0466_C23_C23, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE0463-0466_C23_C23
Probe
MAG3928
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
ctccagcctagtgctcgtcgccttcgccttgctctgctgctacgcctcca
ccgtctcctcccagggtaattcgtctgatggtttcctctcgtgtctgacg
gcgagcatcccccgccagctcgtgttcacgccgagctcgccctcgttcac
gccgctgctcaagtcctccatccggagccccaagttcttcacgcccagca
ccgtgaggccgctgtatattatcacgccgacgaacgcgtcgcacgtgcag
gctgccgtgctgtgcggccgtcggagcgggctgcgcatccgcgtgcgcag
cggcgggcacgactacgagggcctgtcgtaccggtccgtgcgcgccgagt
cgttcgccg
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BM137381
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||