GrainGenes Sequence Report: BM135928
Sequence
BM135928
External Databases
Data at GenBank Data at EMBL Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Sumai3
Clone Library
Wheat Fusarium graminearum infected spike cDNA library
Tissue
Spike
Developmental Stage
Adult plant
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2603_C05_F09ZS Wheat Fusarium graminearum infected spike cDNA library Triticum aestivum cDNA clone WHE2603_C05_F09, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2603_C05_F09
Probe
MAG3904
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequences have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20. No effort was taken; to identify ESTs of fungal origin from this library, thus this EST; could be of wheat or fungal origin.; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made , and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors). Note: Vector: Lambda Uni-ZAP XR, excised phagemid pBluescript SK; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Spikes were sprayed at anthesis with Fusarium graminearum. Total RNA, and poly(A) RNA were prepared and pooled from infected spike at 0, 6, 12, 24, 36 and 48 hours after inoculation, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in G. Muehlbauer lab at the University of Minnesota (Kruger, W.M., Muehlbauer, G.J., Pritsch, C., Vance, C.). The cDNA library should contain genes of both wheat and fungal pathogen origin. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aggagaacaatgggggcttctcagcttctagaactcctaccccaacaatg
gcagcttgtgctattcattctcccactcgtctacctcctcctcctgcaca
tgagccggaacagttccagcggcactaggctgccaccagggccctggaag
ctgcccatcgtcggcaacctgcatcagatcggcccgctgccccaccggag
cctgctgactctcgcaaggcggcacgggccggtgatgatgatgcggctgg
gcacggttccgacggtggtgctgtcctcaccggaggccgcacgcgaggct
ctcaagactcacgacgcanactgctgcggccggtccccctcggccgggcc
gcggctgctgtcctacggctacaaggacgtcatcttctccccctatggcg
actacgtccgcgagatgcgcaagctcttcatcctcgagctgctc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BM135928
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||