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GrainGenes Sequence Report: BM100943

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Sequence
BM100943
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Hv.3540
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf59k09, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
pistil, 1 DPA, no treatment, cv Optic, EBpi01
Tissue
pistil
Developmental Stage
1 DPA
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBpi01_SQ002_G23_R pistil, 1 DPA, no treatment, cv Optic, EBpi01 Hordeum vulgare subsp. vulgare cDNA clone EBpi01_SQ002_G23 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBpi01_SQ002_G23
Probe
baak20d06
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: On Nov 21, 2001 this sequence version replaced gi:17032011.; Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from pistils dissected from developing grains (24 hours post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
ctcattctagtgatttttgtgcccatggcatcacctgattgtgcatgccc
tgaaaattgttgcaggctgccgatggcgtcaagtcgcggctctagatcgt
gtacccattttatgccgtgttctgccttgtccagtccaggtgaaggacat
gacaggagctggaatggttcggatacaatcatatattagatgtacgtaat
aaagtgcacagcaaaaatgctacgtacgtaggttggtttgattgttggtg
ggtgtggcttttttcttttcttctgaaactgctgctgtgtaatgtgttgc
tgtggtctttaccgtcggtgcagtagttttctgcattgtggattcccctg
gcgcactgttgcaacatgtccgcgaaacctgtgtatgtttctacagggcc
agggacagtccgtgcgatccttgggaattctggaaaaaaaaaaaa

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