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GrainGenes Sequence Report: BM099678

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Sequence
BM099678
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC158235
NCBI UniGene
Hv.979
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'CDNA clone: FLbaf97j13, mRNA sequence'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Optic
Clone Library
embryo sac, 4-6 DPA, no treatment, cv Optic, EBes01
Tissue
embryo sac
Developmental Stage
4-6 DPA
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
EBes01_SQ003_O19_R embryo sac, 4-6 DPA, no treatment, cv Optic, EBes01 Hordeum vulgare subsp. vulgare cDNA clone EBes01_SQ003_O19 5', mRNA sequence.
Strain
lab_host DH10B
vulgare
Clone
EBes01_SQ003_O19
Probe
kr61G1214
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: On Nov 21, 2001 this sequence version replaced gi:17030734.; Contact: Waugh R, Marshall DF; Genome Dynamics/Computational Biology; Scottish Crop Research Institute; Invergowrie, Dundee, DD2 5DA, Scotland, UK; Tel: 00 44 1382 562731; Fax: 00 44 1382 562426; Email: est@scri.sari.ac.uk; All sequence has a Phred quality score of 20 or over; Seq primer: M13 reverse.
Note: Vector: pSPORT1; Site_1: Sal I; Site_2: Not I; Non-normalised library, directionally cloned into pSPORT1. Derived from embryo sacs dissected from developing grains (4-6 days post anthesis) in glasshouse grown barley plants. Developed as part of the barley transcriptome resources of BBSRC/SEERAD funded cereal IGF (Investigating Gene Function) project.
DNA
gtcacgccacaccacagagctttgcctccgcagcgatggcgaccaaccgc
cgcctccgcgacctgctcctcctcgccgcggcgctcctgatctccgcctc
ctgcgccgccgccgccgacttcgagtactgcaataagggccgccactatc
cggtgaaggtgagcggcgtggagatctcccccgaccccgtcgtgcgcggc
aagcccgccaccttcaagatctccgcctccacggataaaaccatcaccaa
agggaagctggtggtagatgtgtggtacttcttcttccacgtcgattcag
aaactcatgacttctgtgctgggactccctgtcccgcaaccggcgaattc
gtgctaaccagcgaacagactctgccatctttcaccccaccaggctctta
cagtctccagatgaagctgctgggggacaagaaagaggaactgacctgca
tctccttcggcttcaacatcgggttcatcgcgcctgtcgccactatctga
accaaatatgtgccgcggaccaaacacgcccttggttgcagttgcaaaga
gcacaccacgtttgtatgtatgtacctgcagaactatgtgaactctgaat
caccccgagtgagagagcatgtgttggactgagcctttttattcagattt
gctg

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