GrainGenes Sequence Report: AA231681

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Sequence

AA231681

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'CDNA clone: FLbaf33b21, mRNA sequence'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Willis

Clone Library

cDNA from barley

Tissue

etiolated leaf

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

BCD454.R cDNA from barley Hordeum vulgare subsp. vulgare cDNA clone BCD454, mRNA sequence.

Strain

vulgare

Clone

BCD454

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: McCouch SR; Dept Plant Breeding; Cornell University; Ithaca, NY 14853-1901, USA; Tel: 607 255 0420; Fax: 607 255 6683; Email: srm4@cornell.edu; cDNA from barley (Hordeum vulgare); reverse sequence of RFLP probe; BCD454. Sequence determined by Nicola M. Ayres. For mapping; information, additional citations and other related information; concerning this probe, please refer to the RiceGenes database at; http:

Note: Vector: Lambda Zap II/pBluescript; Site_1: EcoRI; A Lambda ZAP II cDNA library was constructed from mRNA extracted from etiolated leaf tissue of the barley cultivar 'Willis' and converted to pBluescript (amp resistant) as described in Heun et al. (1991) Genome 34:437-447. For insert amplification, Use M13 forward and reverse primers. Clones from this library are designated with the prefix 'BCD'.

DNA Homology

BCD454-5

DNA

cagagataatggttcctcttgttggaacacctcaggaattgggacatcaa
gtggctcttatccgccaaattgctaagaatgttttcaccaatatggggaa
aaccattgattacaaagttgggactatgattgaaattcctagggcagctc
tagtggcagatgagataccagagcaggctgagttcttctctttcggaacg
aacgacctcacgca


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