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GrainGenes Sequence Report: BI960038

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Sequence
BI960038
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Hv.6018
DB Remark
Locus Source: Hordeum vulgare subsp. vulgare
UniGene title 'Transcribed locus, moderately similar to NP_001059468.1 Os07g0417800 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Hordeum vulgare subsp. vulgare
Cultivar
Morex
Clone Library
Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Tissue
Rachis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
HVSMEn0022P11f Hordeum vulgare rachis EST library HVcDNA0015 (normal) Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0022P11f, mRNA sequence.
Strain
lab_host TJC121
vulgare
Clone
HVSMEn0022P11f
Probe
bah34a23
Remark
DB_xref: taxon:112509
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 489; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 534.
Note: Vector: pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown at Washington State University, Pullman, WA in a greenhouse, the rachises were excised and frozen in liquid nitrogen (Kleinhofs lab). In the TJ Close lab at the University of California, Riverside total RNA was prepared, poly(A) was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Chin). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:
DNA
ccgacacacacgcagggcctgggggatgaagcagagctcggacttctgag
aacctcgcacaagtccttgctgtcatgccatgaccagacatcattcgaag
ggatgagcatgacgatgagcttcccgacgaggagccggtgtttttgccgg
ttgaacgacctcaccatgtcgaccgcgtgttccttgacaggtaccaggaa
gaaaagatctgctcgtgccatgtcgaggtcgttcgaccagcaaaacggcg
gcggacttcgtctaccgcaaggcccgcggtgccgacggtggccttccggg
gccgcgggtcaccatgagcttcaaagttatctcctctagcagcggctaga
cggcgtcagctatgatgaagacatgctggctggcaaaacgccccttgccc
ttcctgtttttgagaatgcggatttggttctgcacatgaagtatatggtt
gtgtgtatcacctggatccatggaatacaaagacataggctccgagatga
tacccatgatttgttcgtaaaaaaaaaaaaaaaaaatctt

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