GrainGenes Sequence Report: BI958135

[Submit comment/correction]

Sequence

BI958135

External Databases

TIGR Gene Index

NCBI UniGene

DB Remark

Locus Source: Hordeum vulgare subsp. vulgare

UniGene title 'Transcribed locus, strongly similar to NP_001051032.1 Os03g0707600 [Oryza sativa (japonica cultivar-group)]'

Keyword

EST

Species

Hordeum vulgare subsp. vulgare

Cultivar

Morex

Clone Library

Hordeum vulgare rachis EST library HVcDNA0015 (normal)

Tissue

Rachis

Data Source

genbank	Release 135, Apr 15 2003
genbank	Updated Nov 2006

Title

HVSMEn0013J07f Hordeum vulgare rachis EST library HVcDNA0015 (normal) Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0013J07f, mRNA sequence.

Strain

lab_host TJC121

vulgare

Clone

HVSMEn0013J07f

Probe

baal39e15

Remark

DB_xref: taxon:112509

Feature: source: mol_type = 'mRNA';

Locus Comment: Contact: Wing RA; Clemson University Genomics Institute; Clemson University; 100 Jordan Hall, Clemson, SC 29634, USA; Tel: 864 656 7288; Fax: 864 656 4293; Email: rwing@clemson.edu; Total hq bases = 418; Seq primer: AATTAACCCTCACTAAAGGG; High quality sequence stop: 577.

Note: Vector: pBluescript SK(-); Site_1: EcoRI; Site_2: XhoI; Plants were grown at Washington State University, Pullman, WA in a greenhouse, the rachises were excised and frozen in liquid nitrogen (Kleinhofs lab). In the TJ Close lab at the University of California, Riverside total RNA was prepared, poly(A) was purified, one primary unamplified cDNA library was made, and 1 million pfu were in vivo excised to give pBluescript SK(-) cDNA phagemids (Chin). Phagemids were plated and picked at the Clemson University Genomics Institute (CUGI) (Begum, Palmer, Frisch, Atkins and Wing). Plasmid DNA preparations, DNA sequencing and sequence analysis were performed at CUGI (Wing, Yu, Frisch, Henry, Simmons, Rambo, Main). The sequence has been trimmed to remove vector sequence and contains a minimum of 100 bases of phred value 20 or above. For more details on library preparation and sequence analysis see http:

DNA

tttttttttttttttttttgctaacaaagtccggcccgtgcttatttcaa
tatgagcgattggaggaattacacatagcaatagtaatagacatttaaac
ataataataatagtaatagggcttaataaggagttttacgggtgatggtg
aactgggaacgaaggagggattacattacatgccggcggtctggtcggag
ttggtcgtgtccatgtcttcacgccggacgcagaaccaaaccgtagagtg
gtaacccgatcgatcctagccggtcgctgcaattcagcgtatcactgaca
tttagcttcttcttcatcttcttcttcttcaagtgcgtgtgcgtgcgccg
gagagccggggcgggcgggcggccggggttgtgtcgtancgctccatgct
ctcacgatgtctacttacagcgttcaaaactcgcgatcacggcgcgggga
ggcgccatgcggaagtggcgatcaacgggcgcctgtgccaccgagagtca
ggcacccttgcttctcctccaccttgaacccttgcccgcgggggaaaagg
gccacaagcttgctcgcctggttgtaggctttgaacccaagtggacaaga
aaattcttcacaggacagcctagacagaatggggtccattggggcaggtc
aacaagggcggcgaaagggctgtgtcgggacctccatttgcggggcgaag
gccggcgcgacagccatacgccacgggggcgttgcgattgtacggggagg
actgactgagtttgtgcaggagaggtgggcctggcggggatagatggtgc
ggtctggcatgatatgcagggggtgggcgacgcgggcagacgggaatagt
cgaggaggtcaggacgtggccggcggngagacgaggaagggatgagcn


GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.