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GrainGenes Sequence Report: BI751549

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Sequence
BI751549
Contig
Ta.27139.1.S1_at
Ta.27139.1.S1_x_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC331415
NCBI UniGene
Ta.57833
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Clone wdk1c.pk014.n5:fis, full insert mRNA sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Frontana
Clone Library
Ta01_AAFC_ECORC_Fusarium_graminearum_inoculate d_wheat_heads
Tissue
heads
Developmental Stage
anthesis
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
Ta01_19e12_R Ta01_AAFC_ECORC_Fusarium_graminearum_inoculated_wheat_heads Triticum aestivum cDNA clone Ta01_19e12, mRNA sequence.
Clone
Ta01_19e12
Probe
MAG3569
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Ouellet, Therese; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A 0C6,; CANADA; Tel: (613) 759-1658; Fax: (613) 759-1701; Email: ouellettr@em.agr.ca.
Locus Comment: Contact: Ouellet, Therese; Eastern Cereal and Oilseed Research Centre; Agriculture and Agri-food Canada; Neatby Bldg., Central Experimental Farm, Ottawa, Ontario, K1A 0C6,; CANADA; Tel: (613) 759-1658; Fax: (613) 759-1701; Email: ouellettr@agr.gc.ca.
Note: Vector: pGEM-T easy; Site_1: EcoRI; Site_2: EcoR1; Controled chamber-grown wheat heads were spray inoculated at mid-anthesis with a Fusarium graminearum macroconidial suspension (50,000 spores/ml) and kept under intermitent misting for 24 hours, then collected and immediately frozen in liquid nitrogen.
DNA
acatcaatccatcaacggatagagcaatgtggcacatgattttwtggtag
aaaagcggkcacttcagtagcatttcagcaatctgcacggcgttcagcgc
ggcgcctttacgtatctggtctccacagacaaataactccaaccctctgt
tatcatcttgcgacaggtcctgacgaatcctaccaactgatacatcatct
ttgtccgatacctccagtggtgtggggaagcggttggcagcacggtcgtc
actaatggtwwnaccaggagctgccctcaagatttctctggcagtgtcct
catcaagtggcttttcaaactgtagattcgcgctttcagcgtgtgcacgc
atcgtaggaacccgtatacaagtcgcggttactcttacatccttgtcatt
ccagatttttctggtctccttcaccattttcatctcttcctcattatagc
cattttcaacaataggtgcattatgcgaaaatatattaaaagcatactgt
tgactgaaaatgttgcaggttggtggctttccttccaagacctcttgagt
ttgaagtttgagttcttccatggctgcagcaccagcaccacttgctgctt
ggtatgtgctgacaaccatccttttcaccttggcgtggcgatgcagcggc
gtgacagccatgaggcagatgatggtggaacagttggggttggccacgat
agcccctttcccgagccggacgtcggccatggcctcggggttgacctcgg
ggatgacgagcggcacgtcggggtccatccggt

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