GrainGenes Sequence Report: BI480465
Sequence
BI480465
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC339873
NCBI UniGene
Ta.66022
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001060731.1 Os07g0693600 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
BH1146
Clone Library
Wheat aluminum-stressed root tip cDNA library
Tissue
Root tip at 1.0 to 1.5 mm stage
Developmental Stage
Seedling
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2903_D12_H23ZS Wheat aluminum-stressed root tip cDNA library Triticum aestivum cDNA clone WHE2903_D12_H23, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2903_D12_H23
Probe
CFE281
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors ). Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown under hydroponic conditions, root tips were excised and snap frozen, total RNA was prepared at University of Missouri(Ross, Gustafson). Poly(A) RNA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript SK- phagemids in the TJ Close lab (Chin and Close) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
acacgctggtggaccggccggtggcgtacaaggaggtgctcaagacgtgg
gccaagtgggtggaccggcacgtcgaccccggccggaccaaggtcttctt
catgggcatgtcgcccaaccacggcgtgccggacgcgtggggccgggggg
gccgggagcgatcaagtgcgcgatggagacgcagccgatcctcaaccaca
cgggcccgctctacatcggcaccgactggcggctgcacggcgcggcggag
gcggtgctgcgctccatgcgccgcgtgccggtgcacctcgtcgacatcac
cgcgctctccgagttccgc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BI480465
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||