GrainGenes Sequence Report: TaAffx.40933.1.S1_at
Sequence
BG909152
Contig
TaAffx.40933.1.S1_at
External Databases
Data at GenBank Data at EMBL Data at DDBJ
NCBI UniGene
Ta.39476
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001041793.1 Os01g0109300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
TaLr1174E09R TaLr1 Triticum aestivum cDNA clone TaLr1174E09 5', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1174E09
Probe
CFE233
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 174 row: E column: 09; Seq primer: M13 Reverse. Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 174 row: E column: 09; Seq primer: M13 Reverse. Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
gaattcggcacgaggtctgcactgctgctggacagcattgttgataaaca
cgcaatagatattgaacctgattatctgaaagttatcaaggaaatgatag
ttgcaagctctgacgtctccacagcagaaggtgtgaaggagaagcgtttc
ctttacgacattgttgctaatgggcgcaacggtatagatgttgacaagtt
cgactacattgaccgtgattgcagagcatgtggtattggcagcaatttcc
agcactggaggcttttagaaggtatgcgagtgatgggtgatgaaatatgc
taccctgccaaagactatctgagcatccataaattgttcacaacacgtgc
tgatctgcatcggactgtctatacccatgccaaagtaaagatgatccaga
ggacttttggaagttagatgacacaatcgtcaaaagcatcgaaactgctc
ccaacgatgaactgaagaaagcaaaggaaatcattcaacgtatccgccga
agggagctctacaaggtagcagttctttccagtcgtaatactgttctatt
attttgtctcctcttctgttacatgcttctcagttctcacagctatttgt
gctcaaactgtggatcttacggttatgcacgtgcaatattatttgaatgt
ttttcaatgtgtagctc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: TaAffx.40933.1.S1_at
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||