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GrainGenes Sequence Report: BG908872

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Sequence
BG908872
Contig
Ta.18594.1.S1_s_at
Ta.9364.2.A1_at
Ta.9364.2.A1_s_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Ta.54147
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaLr1171H06F TaLr1 Triticum aestivum cDNA clone TaLr1171H06 3', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1171H06
Probe
CFE256
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 171 row: H column: 06; Seq primer: M13 Forward.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 171 row: H column: 06; Seq primer: M13 Forward.
Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
gctaggaaccacaaaatataaaaggacaactcatgttcattcaacagtaa
aatactcatgggcattcactgggaagttagcaaccttcaaatacttacaa
aaaatttcatttgcttcatcaggacatagccacacacacacacacacaca
cacaaaaaaacttactgcacaaacctaattctaaggagcagcaaccaaaa
cgaaacaggagcactttaggagagtttcacaatcatcaaaggtgcaacca
gctggttgtcgctgaggggctgttaaatctctggtacataaaaaccttgt
tcacatggggctaatcaaataaaagcagctaaccctgaaagcagctgcaa
caaggaaagctcaggaatcgtcaattactcaaatcaactgccacataaac
atacagaacattaaacaggtatcatatgaccacacttcaataacacatag
atatgctcttgaaaaataaaaacgtgtaaatgcattgagagaaattcccc
accctagggatgggcagctttctttgatctatatagtttcacgggtcttc
tcccg

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