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GrainGenes Sequence Report: BG906103

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Sequence
BG906103
Contig
Ta.25436.2.S1_at
Ta.25436.2.S1_x_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC355563
NCBI UniGene
Ta.25436
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, moderately similar to NP_001056701.1 Os06g0132400 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaLr1146B07R TaLr1 Triticum aestivum cDNA clone TaLr1146B07 5', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1146B07
Probe
CFE138
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 146 row: B column: 07; Seq primer: M13 Reverse.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 146 row: B column: 07; Seq primer: M13 Reverse.
Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
gcttgaaccaccgagagattagagataccaccgagagattagagatacac
gtacgtattagctacactctgcaagatacaaccacagtcggtctatgaac
acatttgctacgacgagccggcggcgacggggacggcctcgtagagcttg
gcgaagtagaactgggtggagatgaagcccttgttcttgacgcgccagcc
ggcggcgcggagcgcctcctcgatgtccttctcggagtggaggtaggccc
gcg

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