Query (optional)   in Class  

GrainGenes Sequence Report: BG905673

[Submit comment/correction]

Sequence
BG905673
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
NCBI UniGene
Ta.3800
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus, weakly similar to NP_001061700.1 Os08g0384100 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaLr1141F01R TaLr1 Triticum aestivum cDNA clone TaLr1141F01 5', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1141F01
Probe
CFE31
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 141 row: F column: 01; Seq primer: M13 Reverse.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 141 row: F column: 01; Seq primer: M13 Reverse.
Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
gaattcggcacgagctccagtccgtccgagaccggcatggccaccacggc
cgccgccgccgccgcggtcccatacgctattgctgctctggtgcctgctg
cctcgtccgcacaaccgcataatgttccatacaaatgtactaattataaa
aaccatttgcaagagtggactcaaaggaatgatcagaagttgccatctta
taatactga

GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.