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GrainGenes Sequence Report: BG905481

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Sequence
BG905481
Contig
TaAffx.121290.1.A1_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum aestivum (bread wheat)
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaLr1140A11F TaLr1 Triticum aestivum cDNA clone TaLr1140A11 3', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1140A11
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 140 row: A column: 11; Seq primer: M13 Forward.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 140 row: A column: 11; Seq primer: M13 Forward.
Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
cacctccaatttccaatggaaattacaacaacattaaagaccgcccaggg
gggcgtccaagcgcgcccaacgcggccttatgcatcaaacctcaaactga
accaaaaaaaaaaaccgtccgtagtacaatattaaccgcccacccgaata
ataatcgccccctcccaacaataagtgggggcaaattttgccgggggcca
tgg

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