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GrainGenes Sequence Report: BG904631

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Sequence
BG904631
Contig
Ta.28254.1.S1_s_at
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC290279
NCBI UniGene
Ta.28254
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Myo-inositol 1-phosphate synthase-like mRNA, partial sequence'
Keyword
EST
Species
Triticum aestivum
Cultivar
Thatcher Lr1
Clone Library
TaLr1
Tissue
Leaf tissue
Developmental Stage
14 Days old
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
TaLr1133G03F TaLr1 Triticum aestivum cDNA clone TaLr1133G03 3', mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
TaLr1133G03
Probe
CFE110
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@em.agr.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 133 row: G column: 03; Seq primer: M13 Forward.
Locus Comment: Contact: Dr. Sylvie Cloutier; Cereal Research Centre, Agriculture and Agri-food Canada; 195 Dafoe Rd, Winnipeg, MB, Canada R3T 2M9; Tel: (204) 983-2340; Fax: (204) 983-4604; Email: scloutier@agr.gc.ca; was cloned directionally, not all sequences generated with reverse; primer were from the 5' end (same with forward primer and 3' end).; Average inset size is >2.2 kb; Plate: 133 row: G column: 03; Seq primer: M13 Forward.
Note: Vector: Lambda ZapII; mass excised in plasmid vector pBK-CMV (Stratagene).; Site_1: EcoRI; Site_2: XhoI; mRNA obtained from wheat NIL Thatcher Lr1 24 hours after inoculation with leaf rust pathogen Puccinia triticina race BBB carrying the avirulence gene Avr1.
DNA
ggaaccgatttaaagttccatatactagtagcacacatactactaattaa
cttacaaaacacgaacagagggaggggactaacttagctaacacgaaccc
tcctccttccacataatccaacaccatggagcccatgagaagctaccaag
tcgcagagaccagacagagagccgaaatagtctcgcagggacacacacac
acactacagggtaaacttaaacaagggaaagaatcgcaaaggaaactaaa
gacaagccgccaaataattgcacagccgatctcgccttttttttcctcct
gccctccggtcgccccccgccacttgctacatacgtacggtcgttctcgg
ccctgcgagcttgggccatgctcctaacttgtactccaggatcatgttgt
tctcgggcgccaggccgacgcaggcc

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