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GrainGenes Sequence Report: BG607301

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Sequence
BG607301
Contig
Ta.262.1.S1_at
Ta.262.1.S1_x_at
NSFT03P2_Contig14227
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
DB Remark
Locus Source: Triticum monococcum
Keyword
EST
Species
Triticum monococcum
Cultivar
DV92
Clone Library
Triticum monococcum early reproductive apex cDNA library
Tissue
Early reproductive apex
Developmental Stage
Seven week-old plants
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2493_H01_P01ZS Triticum monococcum early reproductive apex cDNA library Triticum monococcum cDNA clone WHE2493_H01_P01, mRNA sequence.
Strain
lab_host E. coli XLOLR
Clone
WHE2493_H01_P01
Remark
DB_xref: taxon:4568
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova , J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; The tissue, total RNA, and poly(A) RNA were prepared from apex at double-ridge stage to terminal-spikelet stage during transition from vegetative state to flower state, a cDNA library was made, and the cDNA clones were in vivo excised at the University of California, Davis (V. Echenique, B. Stamova, J. Dubcovsky). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
cgttgagtttccttacatcaaggaggaccctttgaagcctacagcggcca
ttgtgaagttctatgttctgtacgaaaagtggcgtagagctgatgttcca
aaggctgattcagttatacagtacttcaaaaacatcacagatgcaaatgg
agttattattcatccgtctggggtcgaactttcgatccattcggccattg
atgagctggcttcatgttatggtgacaaacaagggaaaaagtaccgatca
tgggtggacagactagttatcttgcagactgcttctgacagttggcttgt
gagattcgacttgtgggaggcagaaggtgctgcgtgggtgtgctgtttta
caactcttgcactgaatgtaaagcctgaatcccctggggggttttgtggt
aacacacatccacaagacatggcgtaaagaatatcctggggtcgagcagt
catccaaactctagactggatcgccaccacgggtgcacgggaaggagttc
ttcttgaggatcaaataagaaatcgtagcgaaagcatgtgaatttatctt
acttgttcataatgttgttaataagtgctcccagacgcatatactggact
ggatgatgtctacggttgtctgtctcttgatgc

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