GrainGenes Sequence Report: BG606545
Sequence
BG606545
Contig
Ta.5533.1.S1_at Ta.5533.2.S1_a_at NSFT03P2_Contig4318
External Databases
Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index
TC292033
NCBI UniGene
Ta.10153
DB Remark
Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, moderately similar to NP_001049783.1 Os03g0288300 [Oryza sativa (japonica cultivar-group)]'
Keyword
EST
Species
Triticum aestivum
Cultivar
Brevor
Clone Library
Wheat dormant embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature seed
Data Source
genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title
WHE2957_G02_N03ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2957_G02_N03, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2957_G02_N03
Remark
DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
aatttcagctgacagggcaagattttcactggttgctccccattcttttc
tttcgtcaaggctagcaggaatccctggaatcagctcatcacttctagag
gaatttggggcacgacttgtgacctatgatcttcctggttttggtgaaag
tgatcctcatccaggccgaaatctgaattcttctgcattagacatgcttc
acttggccgatgcccttgggattgtggacaagttctgggttgtaggctat
tctggaggtggcatgcatgcttggagtgctctgcgctacattcctgaccg
ggtggcaggtgcagcaatgttcgcccctatggcaaatccgtatgactcca
aaatgaccaaggatgagaaacgtaaaatttgggacaggtggtcaactaaa
cggaaactaatgcacattttanctcggagatttccatcactattgcgcct
cttctatcacagaagcttcctttctggaaagcaaggacagactgagagtt
ggttatcattgtcaatgggaaagaaggacaaaaccttactggaagctcct
atgttcaacgcattctgggaaaaggatgttgcagagtctgtgcgc
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Sequence Report: BG606545
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||