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GrainGenes Sequence Report: BG606373

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Sequence
BG606373
Contig
NSFT03P2_Contig12728
External Databases
Data at GenBank
Data at EMBL
Data at DDBJ
TIGR Gene Index
TC327144
NCBI UniGene
Ta.54434
DB Remark
Locus Source: Triticum aestivum (bread wheat)
UniGene title 'Transcribed locus'
Keyword
EST
Species
Triticum aestivum
Cultivar
Brevor
Clone Library
Wheat dormant embryo cDNA library
Tissue
Seed embryo
Developmental Stage
Mature seed
Data Source
genbankRelease 135, Apr 15 2003
genbankUpdated Nov 2006
Title
WHE2955_B11_C21ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2955_B11_C21, mRNA sequence.
Strain
lab_host E. coli SOLR
Clone
WHE2955_B11_C21
Probe
CFE106
Remark
DB_xref: taxon:4565
Feature: source: mol_type = 'mRNA';
Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer.
Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA
gctctcgctctctccggctccgctgctgcccgcgcgctcgcccagcggct
ggccccggccaccaggggctacgcggcggcggcctcctccggggcgatga
ggcgcggcgccgcggcggccgcggacgggaaggcggccagggaggccgag
aaggccgcggccgacgcctcgtgggtgcccgaccccgtcaccggccacta
ccgccccgccaaccgcgccgtcggcgccgaccccgccgacctccgcgccg
cgcacctcgggcagacctacgcacgggcttgaaggccacgctcgacgacc
ctcctggccccgccatggaaggaaaactgctatgctacgtaaaaggggaa
gccaggcggggctcgttcgccgagcgcagcgccgcgggcggctggtgtcg
ctggcgcggcttaacgacgcatcaatcatcatcgtcgatcctatactacg
gaggagatgcggcctggatgtttggctgtttcccattgtgtgtgtgtgcg
cgtgcgtgcg

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