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GrainGenes Sequence Report: BG606122

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Sequence	BG606122
Contig	NSFT03P2_Contig1253
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC324976
DB Remark	Locus Source: Triticum aestivum (bread wheat)
Keyword	EST
Species	Triticum aestivum
Cultivar	Brevor
Clone Library	Wheat dormant embryo cDNA library
Tissue	Seed embryo
Developmental Stage	Mature seed
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2960_G03_M06ZS Wheat dormant embryo cDNA library Triticum aestivum cDNA clone WHE2960_G03_M06, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2960_G03_M06
Probe	CFE93
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown to seed maturity under conditions favoring seed dormancy (L. Dohery at K. Walker_Simmons lab, Washington State University, Pullman, WA). Embryos were cut from mature dormant seed (Doherty). Total RNA was prepared from these embryos, polyA was purified, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab at the University of California, Riverside (Chin, Fenton). Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tttcaaatcccccgcctctgcttcttattccctccccatctccaaatttt cggcgaaattttccattcccccacccccacccgcccttcccttcccttcc cccgtcgctctcagccgcgcagcgctatctagggttggggatcgatttgg ggcctagggttaggtcagtttccccatggcggcggcggcggcggcggagg ggtccggggcagagggccgcggcgaggccggcggcggcggcaggcggcag cagcagcagcaggggctggcggagcgccggatgctccggtcccagtacca cgccatgaagagcctcatcaacgaggagagggatgacatggcgaaggagg actccgacaagttcgcctccatcatcacgcaggtggagagcttgcacgag caagtgcagagacccagggagcaagtagcggatgcagaggctctgctgga catcacaagcacgttggtaaaatccgtgaggtcccagtcaagtgaaagaa tcacaccttctgatttcgtaacagcattgctgaagaaattt

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