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GrainGenes Sequence Report: BG604962

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Sequence	BG604962
Contig	Ta.7657.1.S1_s_at Ta.7657.2.S1_a_at Ta.7657.3.S1_a_at NSFT03P2_Contig13647
External Databases	Data at GenBank Data at EMBL Data at DDBJ
TIGR Gene Index	TC289331
NCBI UniGene	Ta.7657
DB Remark	Locus Source: Triticum aestivum (bread wheat) UniGene title 'Transcribed locus, strongly similar to NP_001060917.1 Os08g0129700 [Oryza sativa (japonica cultivar-group)]'
Keyword	EST
Species	Triticum aestivum
Cultivar	Chinese Spring
Clone Library	Wheat pre-anthesis spike cDNA library
Tissue	Spike before anthesis
Developmental Stage	Adult plant
Data Source	genbank Release 135, Apr 15 2003 genbank Updated Nov 2006
Title	WHE2325_E02_I03ZS Wheat pre-anthesis spike cDNA library Triticum aestivum cDNA clone WHE2325_E02_I03, mRNA sequence.
Strain	lab_host E. coli SOLR
Clone	WHE2325_E02_I03
Remark	DB_xref: taxon:4565 Feature: source: mol_type = 'mRNA'; Locus Comment: Contact: Olin Anderson; US Department of Agriculture, Agriculture Research Service, Pacific; West Area, Western Regional Research Center; 800 Buchanan Street, Albany, CA 94710, USA; Tel: 5105595773; Fax: 5105595818; Email: oandersn@pw.usda.gov; Sequence have been trimmed to remove vector sequence and low; quality sequence with phred score less than 20; Seq primer: Stratagene SK primer. Note: Vector: Lambda Uni-ZAP XR, excised phagemid; Site_1: EcoRI; Site_2: XhoI; Plants were grown in the greenhouse. Whole spike with awns trimmed, white, green and yellow anther were collected and total RNA, and poly(A) RNA were prepared, a cDNA library was made, and the cDNA clones were in vivo excised to give pBluescript phagemids in the TJ Close lab (Choi, Close, Fenton) at the University of California, Riverside. Plasmid DNA preparations and DNA sequencing were performed in the OD Anderson lab (all other authors).
DNA	tggcggcagtggcggcggcgagtagaggttccaccattggctagttcgtc tcaaggagtagaattcttacatggtccctgtaaacaggaggggaacacag cctagggctgggatggagtactttgatgctaggcgtaaaccacataatat tgggaaagtcgttgtggccctgctcttcgtagcactctgtatatttgttc tgaagcaatctcctggttttggtggcaatagtgtgttttctcgtcatgaa cctggggttacccatgtcttagtgacaggaggagctggctatattggttc acatgcctcattacagctattaaaagacaactatcgagttaccattgtgg acaatctttctagaggaaataaaggagcagtaaaggttctccaagaatta tttccggagcctgggagacttcaattcatctatgcagatcttggggatca aaaatctgtcaacaagatattttctgaaaatgcatttgatgctgtgatgc actttgcggctgttgcctatgtgggtgagag

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